Journal: Oncogenesis
Article Title: Inhibition of Bruton’s tyrosine kinase as a therapeutic strategy for chemoresistant oral squamous cell carcinoma and potential suppression of cancer stemness
doi: 10.1038/s41389-021-00308-z
Figure Lengend Snippet: A IHC staining of BTK expression on OSCC tissues in CCRT-resistant (upper) and treatment-naïve tissue, normal tonsil as a control (bottom). Black arrow indicates the immune cells. B Q-score of BTK expression in TSGH patients with OSCC with treatment-naïve ( n = 52) and CCRT-resistant tissues ( n = 18). C Kaplan–Meier curves showing the effect of low- and high-BTK expression on the overall survival of TSGH patients with OSCC. D Overall survival of TSGH patients with OSCC relapses from CCRT. Median gene expression was used to determine low/high cut-off value. E Graphical representation of BTK expression in OSCC patients’ samples with or without additional RT/chemotherapy (top image: BTK expression in patient tissue, bottom image: BTK mRNA expression in patient tissue; rBTK: BTK recombinant protein standard, 77 kDa). F Kaplan–Meier curves showing the effect of low- and high-BTK expression on the overall survival of TCGA patients with OSCC ( n = 269). G Overall survival of TCGA patients with OSCC without additional therapy. H Overall survival of TCGA patients with OSCC relapses from CCRT. Median gene expression was used to determine low/high cut-off value. * p < 0.05, ** p < 0.01.
Article Snippet: The purified standard BTK (C481S, Human, full-length recombinant, MW: 77 kDa, Promega, Wisconsin, USA) as loading control.
Techniques: Immunohistochemistry, Expressing, Control, Gene Expression, Recombinant